Class re-do

VDSers,

Ok – we will shoot for 4:30 on Thursday for Group Meeting Re-do.
We’ll try to get our normal classroom. Otherwise we will try some of the classroom directly above it – 3rd floor of Welch – or the 4th floor conference room in Welch.

For the newer guys – you need to start your PCR for pNIC-Bsa4 cloning. Which means you need to do the Primer design first.
I don’t think this will be obvious without some guidance – so try to see me tomorrow or the mentors to get help.
Once you have submitted a solution for the primer design. You can begin the PCR.
However, you will need to have me or one of the mentors discuss it with you first – since it can be a little tricky.

thanks,
Dr. B

Class cancelled

VDSers – class is cancelled due to the events on campus today.
I would like to schedule another time to do group meeting.

Here is a Doodle link to select the best time.
http://www.doodle.com/ci3zgu66nwapb92b

We will meet to

talk about next steps in research
I will hand back paper copy revisions of the Research Report
and answer questions on the Research Report
Journal club discussion

Wed:  4:30 PM      5:30 PM      6:30 PM
OR

Thu: 4:30 PM      5:30 PM      6:30 PM

Midi-Prep

This past week included a successful Midi-Prep that ended with a below-average concentration of Zhang (128.7 nanograms/microliter; average would be approx. 1200 ng/ul). This loss of concentration was most likely attributed to methods practiced or an oversight. Human error was probably to blame because of the huge difference in concentrations of the final sample and sample 1. Sample 1 was taken after the second centrifuge of the supernatant (surviving shaking, Buffer P3, and centrifuge prior) in step 8 of the slow MidiPrep kit. Simple errors could have been discarding a pipette tip that was used to physically mix the DNA/buffer or ethanol, even though I was attentive during this. What a waste of DNA it just stayed in the tip (doubtful).

Sample 1

Final MidiPrep