Labeling plasmid DNA stocks

After midiprep or maxiprep, you want to label your tubes of stock DNA well because you spent a lot of time and effort making it. Also, this DNA will be shared by the whole lab so it should be well labeled.

Label the top and side of tube – use the red sticky dots if necessary (the white squares are lame)

Put:      Initials, Date, type of DNA (pNIC-Bsa4, pGBR22, HSC0041026, etc. ), the concentration in λ ( lambda = micrograms/microliter )    OR  put the concentration  in nanograms/microliter. If it contains a gene inside of a vector – you should put the Gene name and the Vector name (e.g.     DHFR in pNIC-Bsa4)

Resituation and Starting Midiprep

Happy first week back everyone! This week wasn't too eventful but
involved some vital planning for the next few weeks for me. Upon
returning to UT, I was surprised to hear that class was canceled
because Dr.B's son was on the way(congratulations!). But,
nonetheless, the research had to go on. On Wednesday,I added
glycerol to my pNIC-Bsa4 + CA7 protein for long term storage and
double checked its concentration - 1.36 mg/ml (29.147 uM, calculated
using Beer's Law). Additionally, I verified the presence of the
master plate from which I originally got my protein and ensured
correct labeling and storage. On Wednesday, I also deliberated
which direction to go in - repeat my enzyme assay, grow up more
protein, or Midiprep the protein I already had. Initially, I planned
to grow up more protein but, realizing the difficulty in stopping
over the Labor Day weekend, I talked to super mentor Adam and
decided to Midiprep instead. On Thursday,I began the Midiprep and
started the overnight culture of my pNIC-Bsa4 with CA7. On Friday,
I decanted and spun down my overnight culture and stored it in the
-20C fridge over the weekend. Also, I organized my notebook and
made sure everything was up to date. I plan to continue my Midiprep
next week, alongside performing the refreshers Dr. B has made and
beginning my virtual screening (potentially).