Week 4 Update – Virtual Screening Has Begun…Sort Of.

Hey everyone!

This week I was supposed to be working on my scaled up protein expression but, sadly, it didn’t work out. Everything began normally on Monday and I started my overnight culture but then Tuesday’s unforeseen events overtook us all and my expression was lost. Instead of mourning over my lost colonies, however, I decided to postpone the expression until this week and begin on the virtual screening of my target, CA7. I begin on Wednesday and the first run lasted until Friday night. I just started my second run from home and am excited for the results! I’ve included an image of my bestranking.lst file from my first run, displaying the top-ranking compounds. I also spent time working on the final draft of my report and preparing for the coming week. This week I will continue the virtual screening of my target (and hopefully get some great results), work on my report, and, of course, perform that ominous goliath known as scaled-up expression. I hope everyone has a great week and good luck in the lab. See you there!

Fig 1. Excel results of bestranking.lst file after first run with CA7 (H9 library)