Kan+Sucrose Plates Test

Because Kim got a sort of weird colony on her Kan+Sucrose plates, I decided to run a test with one of the new plates to make sure it that there wasn’t something wrong with them. Kim picked a colony from an old Kan+Sucrose plate and grew it up in 5mL of LB+Kan. Once some bacterial growth was seen, some of the solution was transferred onto one of the new Kan+Sucrose plates, spread, and allowed to grow overnight. This morning, when I checked the plate, there was colony growth (see picture). This result leads me to believe that these plates were made correctly and can be used.