Radhika and Yiling

When you guys start your purification – the first thing to do is start thawing out the samples in a beaker of room temp water. The tubes should be in the -20decC

Then add 1ml of Ni-NTA mix to each tube and incubate for 45 minutes on ice (inverting frequently)

Remember the Ni-NTA mix only 50% Ni-NTA – so technically you end up with 0.5 ml of Ni-NTA beads.

I may be running late – so just call my phone if you have any questions.

Dr. B


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