pgbr22 and pNIC-Bsa4 Midi-prep


Pgbr22 and pNIC-Bsa4 were midi-prepped and the final products were stored in a green microcentrifuge rack in the -20 refrigerator. The concentrations have not been measured yet. There are four 50 ml purple capped tubes in the -20 refrigerator (2 with green tape and 2 with orange tape). These still need to be midi-prepped. For whoever is doing the midi-prep, combine the same colored tubes with each other (so two final tubes-one green and one orange).  Start on “Step 4” of the protocol and follow the directions with the blue triangle.  “P1” and “P3” are in the 4C VDS fridge. Samples do not need to be collected.

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