What did the left eye say to the right eye? Between us, something smells.

I just realized that I actually DRAFTED this post instead of publishing it, I’m dumb. Anyways, this post is long over due, and is for last week:

So, I did another enzyme activity assay and got the results up above. The X axis shows the concentration of the enzyme and the Y axis shows the absorbance readings. Although these results aren’t ideal (something was wrong with sample 6), they’re decent. If you look at the graph, you can see a leveling off of the curving line. This leveling off starts at sample 5. SO a concentration of 0.625 of enzyme will be used.

The next step was the varying substrate assay, which I did about 50 times.

Here are the results that were actually decent. The top two are trials 4 and 5. The bottom graph is the average- without the standard deviation- which I will put in soon. Dr. B and I looked at the results and decided that a 5mM concentration would be best- even though my terrible data says otherwise. In other papers, this is the concentration that is typically used.

Next up is the DMSO assay, which I’ll start on next week.


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