Inhibition Assays

VDSers – the inhibition assay protocols are posted. Yeah – the last experiments!

You should complete the other Enzyme Assays (vary enzyme, vary substrate, vary DMSO) before proceeding to the inhibitors.If you are still working on protein characterization – I would strongly urge you to being your assays and come back to the characterization steps.

Also, you show your enzyme assay results to Dr. B (electronically – blog preferably) before proceeding to inhibition.

The inhibitors are expensive and are common stock (i.e. we will all share them). So, it is important that these be diluted properly. Also the Inhibition protocol is a little different in that it requires a pre-incubation.


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